Nchọpụta nke Oxalic Acid na Mkpụrụ osisi na Akwụkwọ nri site na High-Performance Liquid Chromatography (HPLC)
1. Ụkpụrụ Nnwale na Nhazi Usoro
Dịka asịd organic a na-ahụkarị na mkpụrụ osisi na akwụkwọ nri, ọdịnaya oxalic acid na-emetụta kpọmkwem uto na uru nri. Nnwale a na-eji chromatography mmiri mmiri dị elu (RP-HPLC) agbanwe agbanwe. N'okpuru ọnọdụ usoro mkpanaaka acidic, a na-ekewapụ oxalic acid site na ihe ndị na-egbochi ya site na iji kọlụm chromatographic C18. A na-eji ihe nchọpụta ultraviolet nke edobere na 210 nm maka nyocha ọnụọgụgụ, dabere na njirimara nnabata UV nke otu carboxyl na molekul oxalic acid.
2. Nkwadebe Ụkpụrụ na Nlereanya Ihe Nlereanya
A na-eji usoro ịgbaze mmiri nke gradient eme ihe iji kwadebe ngwọta ọkọlọtọ: tụọ 25.0 mg nke oxalic acid dihydrate nke ọma wee gwakọta ya ruo 25 mL na mmiri ultrapurure iji nweta ngwọta ngwaahịa 1 mg/mL. A na-agbaze nke a n'usoro n'ime usoro ngwọta ọkọlọtọ na 50, 100, 200, 400, na 800 µg/mL, a na-agbakwa ntinye ọ bụla n'ime atọ.
Maka nkwadebe ihe nlele, a na-eji mwepụta enyere aka na ngwa ndakwa nri: tụọ 5.00 g nke mkpụrụ osisi/akwụkwọ nri homogenate, tinye 10 mL nke 0.1 mol/L mmiri hydrochloric acid, gwọọ ya na 60°C na ngwa ndakwa nri maka nkeji iri, sachaa ya site na nzacha akpụkpọ ahụ nke 0.45 µm, wee chịkọta nzacha ahụ maka nnwale.
3. Nhazi nke Ọnọdụ Chromatographic
Usoro mkpanaka bụ sistemụ potassium dihydrogen phosphate buffer 0.01 mol/L (pH 2.5)–acetonitrile (95:5), yana ọnụego mmiri nke 0.8 mL/min na okpomọkụ kọlụm nke 35°C. Ịgbanwe oke acetonitrile na-egosi na mgbe usoro organic gafere 10%, oge nchekwa nke oxalic acid na-ebelata ruo ihe na-erughị nkeji 3, mana oke ọdụ na-eme. N'okpuru ọnọdụ kachasị mma, oge nchekwa nke oxalic acid bụ nkeji 4.2, na-enweta nkewa zuru oke site na citric acid na malic acid dị nso (mkpebi > 1.5).
4. Nnwale Usoro
Nnwale oke ahịrị na-egosi ezigbo mmekọrịta dị n'etiti mpaghara kachasị elu na ntinye n'etiti 10-1000 µg/mL (R² = 0.9993). Oke nchọpụta (LOD), nke usoro nha mgbaàmà-na-mkpọtụ kpebiri, bụ 0.5 µg/mL. Na ule imegharị, RSD maka mkpebi ugboro isii nke otu ihe nlele spinach bụ 1.8%. Nnwale mgbake Spike na ọkwa atọ (80%, 100%, na 120%) na-enye nkezi mgbake nke 98.2%, 102.4%, na 97.8%, n'otu n'otu, na-emezu ihe achọrọ maka nyocha ọnụọgụ.
5. Nyocha nke Ezigbo Ihe Nlereanya
A nwalere mkpụrụ osisi na akwụkwọ nri isii dị maka azụmaahịa: akwụkwọ nri spinach (356 ± 12 mg/100 g), celery (215 ± 9 mg/100 g), tomato (18 ± 2 mg/100 g), apụl (6 ± 1 mg/100 g), unere (achọpụtaghị), na broccoli (89 ± 5 mg/100 g). Ntụnyere ya na usoro ọkọlọtọ mba (GB 5009.277-2016) na-egosi na ọdịiche dị n'etiti usoro abụọ ahụ erughị 5%, na-egosi ntụkwasị obi nke usoro a. Karịsịa, usoro HPLC na-egosipụta mmetụta dị elu karịa usoro titration ọdịnala mgbe a na-enyocha ihe nlele ọdịnaya dị ala.
6. Ihe Ndị Dị Mkpa
Jikwaa pH nke ọma n'oge a na-eme ihe nlele tupu a gwọọ ya. Ọ bụrụ na pH nke ihe a na-ewepụta gafere 3, mmiri ozuzo calcium oxalate ga-ebute obere nsonaazụ nnwale. A ga-akwadebe usoro mkpagharị ahụ ọhụrụ kwa ụbọchị iji zere mmiri ozuzo na mkpọchi kọlụm. Mgbe agbachara ọgwụ iri abụọ ọ bụla, jiri acetonitrile-mmiri sachaa kọlụm ahụ ruo nkeji iri atọ iji gbochie mmebi arụmọrụ kọlụm. Maka ihe nlele nwere agba (dịka ọmụmaatụ, kabeeji odo odo), a na-atụ aro ka ị tinye usoro nhicha siri ike tupu a gbaa ya ọgwụ.
7. Ntuziaka maka itinye na ịgbasa
Enwere ike ịgbatị usoro a iji chọpụta oxalic acid n'ime ihe nlele dị ndụ dịka mmamịrị na ọbara. Site n'ịgbanwe nhazi nke usoro mkpanaka (dịka ọmụmaatụ, itinye tetrabutylammonium hydroxide), enwere ike ịchọpụta oxalic acid na metabolites ya n'otu oge. Ejikọtara ya na ihe nchọpụta mass spectrometry, enwere ike ịmepụta usoro nchọpụta trace ka mma. N'ime ụlọ ọrụ nhazi nri, usoro a nwere ike inye nkwado data dị mkpa maka nhọrọ nke ụdị acid dị ala na-enweghị oxalic na imeziwanye usoro esi nri.
Zibo Anhao Chemical Co., Ltd. – Oxalic Acid
Zibo Anhao Chemical Co., Ltd. bụ onye a pụrụ ịtụkwasị obi na-ebubata ma na-ebupụ oxalic acid dị elu (ọkwa ụlọ ọrụ, nkeji 99.6%) site na Shandong, China. Oxalic acid anyị bụ kristal ọcha siri ike, nke a makwaara dị ka ethanedioic acid, nwere usoro molekul C₂H₂O₄. Ọ nwere ịdị ọcha dị elu, adịghị ọcha, na ịdị mma kwụsiri ike n'ọtụtụ otu, na-emezu ihe achọrọ nke ákwà, akpụkpọ anụ, ígwè, nhazi ala dị ụkọ, nhicha kemịkalụ, na ụlọ ọrụ owuwu.
Anyị na-enye ma acid oxalic nke na-anaghị amịpụta mmiri na nke na-adịghị amịpụta mmiri, nke dị na akpa 25kg, akpa 50kg, na akpa 1000kg buru ibu. Site na usoro QC siri ike, akụkọ nnwale zuru oke, na atụmatụ ọkọnọ na-agbanwe agbanwe, anyị na-ahụ na nnyefe kwụsiri ike na arụmọrụ na-aga n'ihu maka ndị na-azụ ahịa n'ụwa niile. A na-ebuga ngwaahịa anyị n'ebe ndịda ọwụwa anyanwụ Eshia, Yuropu, Afrịka, na Amerịka, na-enweta nkwanye ùgwù maka ịdị mma a pụrụ ịdabere na ya na ọnụ ahịa asọmpi.
Họrọ Zibo Anhao Chemical maka inye oxalic acid a pụrụ ịdabere na ya na ọrụ kemịkalụ ọkachamara nke otu nkwụsị.
Họrọ Zibo Anhao Chemical maka oxalic acid dị ọcha nke na-enye arụmọrụ na uru kwụsiri ike maka azụmahịa gị. Kpọtụrụ anyị maka iwu buru ibu ma ọ bụ nkọwapụta teknụzụ: info@anhaochemical.com
